The CELLine Classic bioreactor flask, specifically the CL 350 model designed for 350mL working volumes, represents a significant advancement in cell culture technology. This versatile and relatively compact system offers a compelling alternative to traditional cell culture methods, providing a controlled environment for high-density cell growth and consistent product yields. This detailed guide explores the CL 350's design, functionality, applications, advantages, limitations, and practical considerations for researchers utilizing this powerful tool.
I. Design and Functionality:
The CELLine CL 350 is a single-use, disposable bioreactor flask featuring a unique, patented design. Unlike traditional shake flasks or stirred tank bioreactors, the CL 350 employs a gas-permeable membrane for oxygen transfer and a separate cell culture chamber. This innovative configuration allows for high cell densities without the need for complex stirring mechanisms or potentially damaging shear forces. The 350mL working volume is optimized for a wide range of cell types and experimental scales, making it suitable for both research and small-scale production applications.
The key components of the CL 350 include:
* Cell Culture Chamber: This chamber houses the cells and the culture medium. Its design promotes even distribution of nutrients and oxygen, ensuring uniform cell growth throughout the flask. The surface area of the chamber is optimized to maximize cell growth while minimizing the risk of cell aggregation.
* Gas-Permeable Membrane: This membrane, typically made of silicone, allows for the diffusion of oxygen and carbon dioxide between the cell culture chamber and the surrounding environment. This passive oxygenation eliminates the need for complex aeration systems, simplifying the setup and operation of the bioreactor. The membrane's permeability is carefully controlled to maintain optimal gas exchange while preventing contamination.
* Medium Reservoir: A separate reservoir holds the culture medium, which is gradually fed into the cell culture chamber as needed. This controlled feeding strategy helps to maintain consistent nutrient levels and prevents nutrient depletion, which can negatively impact cell growth and product yield.
* Sampling Port: The CL 350 typically includes a sampling port that allows for the non-destructive removal of small aliquots of the culture medium for analysis. This allows researchers to monitor cell growth, nutrient consumption, and product formation without disturbing the culture significantly.
* Cell Harvesting Port: A designated port allows for efficient and sterile harvesting of the cell culture at the end of the experiment. This feature minimizes contamination risks and simplifies the downstream processing steps.
II. Applications:
The versatility of the CELLine CL 350 makes it applicable to a broad range of cell culture applications, including:
* Mammalian Cell Culture: The CL 350 is particularly well-suited for the cultivation of mammalian cells, including adherent and suspension cell lines. Its gentle mixing and controlled environment minimize shear stress, improving cell viability and productivity.
* Insect Cell Culture: The system can be successfully used for the cultivation of insect cells, which are frequently employed for the production of recombinant proteins.
* Hybridoma Culture: The production of monoclonal antibodies using hybridoma cell lines is another important application of the CL 350. The system's controlled environment and efficient oxygen transfer support high antibody yields.
* Stem Cell Culture: The gentle mixing and controlled conditions offered by the CL 350 make it suitable for the culture of sensitive stem cells, which are crucial in regenerative medicine research.
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